| Identification |
|---|
| BMDB Protein ID
| BMDBP00053 |
| Secondary Accession Numbers
| |
| Name
| Cholesterol side-chain cleavage enzyme, mitochondrial |
| Synonyms
|
Not Available
|
| Gene Name
| CYP11A1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Secondary metabolites biosynthesis, transport and catabolism |
| Specific Function
| A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:11412116). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:11412116). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:11412116). |
| Pathways
|
- C21-steroid hormone metabolism
- Lipid metabolism
|
| Reactions
|
| 3a,7a-Dihydroxy-5b-cholestane + 3 NADPH + 3 Oxygen → 3 alpha,7 alpha,26-Trihydroxy-5beta-cholestane +3 NADP +3 Water |
details
|
| 3 alpha,7 alpha,26-Trihydroxy-5beta-cholestane → 3a,7a-Dihydroxy-5b-cholestan-26-al |
details
|
| 3a,7a-Dihydroxy-5b-cholestan-26-al → 3a,7a-Dihydroxycoprostanic acid |
details
|
| 5-b-Cholestane-3a ,7a ,12a-triol + 3 NADPH + 3 Oxygen → 27-Deoxy-5b-cyprinol +3 NADP +4 Water |
details
|
| 27-Deoxy-5b-cyprinol + 3 NADPH + 3 Oxygen → 3a,7a,12a-Trihydroxy-5b-cholestan-26-al +3 NADP +4 Water |
details
|
| 3a,7a,12a-Trihydroxy-5b-cholestan-26-al → Coprocholic acid |
details
|
| 27-Hydroxycholesterol + 3 NADPH + 3 Oxygen → 3beta-Hydroxy-5-cholestenoic acid +3 NADP +4 Water |
details
|
|
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 21 |
| Locus
| Not Available |
| SNPs
| CYP11A1 |
| Gene Sequence
|
>1563 bp
.AGCAGCAGCTGTGGGGGCAGCATGCTAGCAAGGGGGCTTCCCCTCCGCTCAGCCCTGGT
CAAAGCCTGCCCACCCATCCTGAGCACAGTGGGGGAGGGCTGGGGCCACCACAGGGTGGG
CACTGGAGAGGGAGCTGGCATCTCCACAAAGACCCCTCGCCCCTACAGTGAGATCCCCTC
CCCTGGTGACAATGGCTGGCTTAACCTCTACCATTTCTGGAGGGAGAAGGGCTCACAGAG
AATCCACTTTCGCCACATCGAGAACTTCCAGAAGTATGGCCCCATTTACAGGGAGAAGCT
TGGCAATTTGGAGTCAGTTTATATCATTCACCCTGAAGACGTGGCCCATCTCTTCAAGTT
CGAGGGATCCTACCCAGAGAGATATGACATCCCGCCCTGGCTGGCCTATCACCGATATTA
TCAGAAACCCATTGGAGTCCTGTTTAAGAAGTCAGGAACCTGGAAGAAAGACCGGGTGGT
CCTGAACACGGAGGTGATGGCTCCAGAGGCAATAAAGAACTTCATCCCACTGCTGAATCC
AGTGTCTCAGGACTTCGTCAGCCTCCTGCACAAGCGCATCAAGCAGCAGGGCTCCGGAAA
GTTTGTAGGGGACATCAAGGAAGACCTGTTTCACTTTGCCTTTGAGTCCATCACCAATGT
CATGTTTGGGGAGCGCCTGGGGATGCTGGAGGAGACAGTGAACCCCGAGGCCCAGAAGTT
CATTGATGCCGTCTACAAGATGTTCCACACCAGTGTCCCTCTGCTCAACGTCCCTCCAGA
ACTGTACCGTCTATTCAGAACCAAGACTTGGAGGGACCATGTAGCCGCATGGGACACAAT
TTTCAATAAAGCTGAAAAATACACTGAGATCTTCTACCAGGACCTGAGACGGAAAACAGA
ATTTAGGAATTACCCAGGCATCCTCTACTGCCTCCTGAAAAGTGAGAAGATGCTCTTGGA
GGATGTCAAGGCCAATATTACGGAGATGCTGGCAGGGGGTGTGAACACGACATCCATGAC
ATTGCAATGGCACTTGTACGAGATGGCACGCAGCCTGAATGTGCAGGAGATGCTGCGGGA
GGAGGTTCTGAATGCCCGACGCCAGGCAGAGGGAGACATAAGCAAGATGCTGCAAATGGT
CCCACTTCTCAAAGCTAGCATCAAGGAGACGCTGAGACTCCACCCCATCTCCGTGACCCT
GCAGAGATACCCTGAAAGTGACTTGGTTCTTCAAGATTACCTGATTCCTGCCAAGACACT
GGTGCAAGTGGCCATCTATGCCATGGGCCGAGACCCTGCCTTCTTCTCCAGTCCGGACAA
GTTTGACCCAACCAGGTGGCTGAGTAAAGACAAAGACCTCATCCACTTCCGGAACCTGGG
CTTTGGCTGGGGAGTGCGGCAGTGCGTGGGCCGGCGGATCGCCGAGCTGGAGATGACCCT
CTTCCTCATCCACATTCTGGAGAACTTCAAGGTTGAAATGCAGCATATCGGTGACGTGGA
CACCATATTCAACCTCATCCTGACGCCGGACAAGCCCATCTTCCTTGTCTTCCGCCCCTT
CAA
|
| Protein Properties |
|---|
| Number of Residues
| 520 |
| Molecular Weight
| 60333.0 |
| Theoretical pI
| 9.15 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Cholesterol side-chain cleavage enzyme, mitochondrial
MLARGLPLRSALVKACPPILSTVGEGWGHHRVGTGEGAGISTKTPRPYSEIPSPGDNGWL
NLYHFWREKGSQRIHFRHIENFQKYGPIYREKLGNLESVYIIHPEDVAHLFKFEGSYPER
YDIPPWLAYHRYYQKPIGVLFKKSGTWKKDRVVLNTEVMAPEAIKNFIPLLNPVSQDFVS
LLHKRIKQQGSGKFVGDIKEDLFHFAFESITNVMFGERLGMLEETVNPEAQKFIDAVYKM
FHTSVPLLNVPPELYRLFRTKTWRDHVAAWDTIFNKAEKYTEIFYQDLRRKTEFRNYPGI
LYCLLKSEKMLLEDVKANITEMLAGGVNTTSMTLQWHLYEMARSLNVQEMLREEVLNARR
QAEGDISKMLQMVPLLKASIKETLRLHPISVTLQRYPESDLVLQDYLIPAKTLVQVAIYA
MGRDPAFFSSPDKFDPTRWLSKDKDLIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILE
NFKVEMQHIGDVDTIFNLILTPDKPIFLVFRPFNQDPPQA
|
| External Links |
|---|
| GenBank ID Protein
| AAA30488.1 |
| UniProtKB/Swiss-Prot ID
| P00189 |
| UniProtKB/Swiss-Prot Entry Name
| CP11A_BOVIN |
| PDB IDs
|
|
| GenBank Gene ID
| K02130 |
| GeneCard ID
| CYP11A1 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
