| Identification |
|---|
| BMDB Protein ID
| BMDBP00460 |
| Secondary Accession Numbers
| None |
| Name
| Polyribonucleotide 5'-hydroxyl-kinase Clp1 |
| Synonyms
|
Not Available
|
| Gene Name
| CLP1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in ATP binding |
| Specific Function
| Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 15 |
| Locus
| Not Available |
| SNPs
| CLP1 |
| Gene Sequence
|
>1278 bp
ATGGGAGAGGAGGCCAATGATGACAAGAAGCCAACAACTAAATTTGAACTGGAGCGGGAG
ACAGAGCTTCGCTTTGAGGTGGAGGCGTCGCAGTCAGTTCAGTTGGAGCTGCTGGCTGGC
ATGGCAGAAATCTTCGGCACAGAGCTGACCCGAAACAAGAAGTTCACCTTTGATGCCGGC
GCCAAGGTGGCTGTCTTCACTTGGCACGGCTGTTCGCTGCAGCTCAGTGGCCGCACCGAG
GTGGCTTACGTCTCCAAGGATACCCCAATGTTGCTTTATCTCAACACGCATACGGCCTTG
GAGCAGATGCGGAGGCAGGCAGAGAAGGAGGAGGAGCGAGGCCCCCGGGTGATGGTCGTG
GGCCCCACGGACGTGGGCAAGTCCACCGTGTGCCGTCTGCTGCTCAACTACGCGGTGCGT
TTGGGCCGCCGGCCCACGTACGTGGAGCTGGATGTGGGCCAGGGCTCTGTGTCCATCCCC
GGCACCATGGGGGCCCTGTACATTGAGCGGCCAGCGGACGTTGAGGAGGGGTTCTCCATC
CAGGCCCCGCTGGTGTACCACTTCGGCTCCACCACGCCTGGCACCAACATCAAGCTTTAT
AATAAGATTACATCTCGTTTAGCCGATGTGTTCAACCAGAGGTGTGAAGTGAACCGCAGG
GCCTCGGTGAGCGGCTGTGTCATTAACACCTGTGGCTGGGTCAAGGGCTCTGGTTACCAG
GCCCTGGTCCATGCAGCCTCAGCCTTTGAGGTGGATGTCGTTGTGGTTCTGGATCAAGAA
CGGCTGTACAATGAACTGAAACGGGACCTGCCTCACTTCGTCCGCACGGTGCTGCTCCCC
AAGTCGGGGGGTGTGGTGGAGCGCTCCAAGGACTTCCGGCGGGAATGCCGGGATGAGCGC
ATCCGTGAGTATTTCTATGGGTTCCGGGGCTGTTTCTATCCCCATGCCTTCAACGTCAAA
TTTTCAGATGTGAAAATCTACAAAGTCGGGGCACCCACCATCCCAGACTCTTGCCTGCCT
CTGGGCATGTCTCAGGAAGACAATCAGCTCAAGCTGGTGCCTGTTACCCCCGGCCGAGAT
ATGGTGCACCACCTGCTGAGCGTCAGCACGGCGGAGGGCACAGAGGAGAACCTCTCCGAG
ACCAGCGTGGCGGGCTTCATCGTGGTGACCAGTGTGGACCTGGAGCACCAGGTGTTCACC
GTTCTCTCTCCGGCCCCCCGCCCACTGCCCAAGAACTTCCTTCTCATCATGGATATCCGG
TTCATGGATCTTAAGTAG
|
| Protein Properties |
|---|
| Number of Residues
| 425 |
| Molecular Weight
| 47630.0 |
| Theoretical pI
| 6.6 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Polyribonucleotide 5'-hydroxyl-kinase Clp1
MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLAGMAEIFGTELTRNKKFTFDAG
AKVAVFTWHGCSLQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVV
GPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSI
QAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQ
ALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDER
IREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRD
MVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIR
FMDLK
|
| External Links |
|---|
| GenBank ID Protein
| AAI33500.1 |
| UniProtKB/Swiss-Prot ID
| A2VE01 |
| UniProtKB/Swiss-Prot Entry Name
| CLP1_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC133499 |
| GeneCard ID
| CLP1 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
