1.0 2018-05-07 22:25:12 UTC 2020-05-20 20:59:40 UTC BMDBP01051 Enzyme TOP3A Replication, recombination and repair Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. BMDB0001303 Zinc 19 >3009 bp ATGATATTTCCGGCCGCCCACCGCGCTCTCCGGGGGCTGCCGCGGCCCGGTACTCGGGCC CTGACTCGCGCCGCCATGGAGGTGGCCCTGCGAGGCGTGCGGAAAATCCTTTGCGTGGCC GAGAAGAACGACGCGGCCAAGGGCATAGCCGACTTGCTGTCTGGCGGCCGCATGCGGCGG AGGGAAGGACTTTCAAGATTCAACAAGATCTATGAGTTTGATTATCATCTGTGTGGCCAG AATGTCACCATGGTAATGACCTCAGTCTCAGGACACTTGCTGGCCCATGATTTCCGGATG CAGTTTCGCAAATGGCAGAGCTGCAATCCTCTTGTCCTGTTTGAAGCAGAAATTGAAAAG TACTGCCCAGAGAATTTTGTAGACATCAAGAAAACTCTGGAGCGAGAGGCCCAGCAATGC CAGGCCCTGGTGATTTGGACCGACTGCGACAGAGAGGGTGAAAACATTGGGTTTGAGGTC ATCCACGTGTGCAAGGCCGTGAAGCCCAGCCTGCAGGTGCTGCGAGCCCGTTTCTCCGAG ATCACCACCCGCGCTGTCCGGACGGCATGTGAAAACCTTACGGAGCCCGACCAGAGAGTG AGCGATGCCGTGGATGTGAGGCAGGAGTTGGACCTGCGGATCGGGGCGGCCTTCACCAGG TTCCAGACGCTGCGGCTGCAGAAGATCTTCCCCGAGGTGCTGGCAGAGCAGCTTATCAGC TACGGCAGCTGCCAGTTCCCCACCCTGGGGTTCGTGGTGGAGAGGTTCAAAGCCATTCAG GCATTTGTGCCAGAAGTCTTCCACAAGATCCGAGTGACTCATGACCACAGGGACGGCGTG GTAGAGTTCAGCTGGAAAAGGCATCGTCTCTTCAATCACACGGCATGTCTCGTCCTCTAC CAGATGTGCATGGAGGATCCCAGGGCGATGGTGGTAGAGGTCAGGTCAAAAGCCAAGAGC AAGTGGAGGCCTCAGGCTCTGGACACTGTGGAGCTGGAGAAGCTGGCATCTCGGAAGTTG AGGATAAATGCTAAAGAAACCATGAGGATTGCAGAAAAGCTCTACACCCAAGGGTACATC AGCTATCCTCGAACTGAAACGAACATTTTCCCCAAAGACCTAGACCTGGCGGCACTGGTG GAGCAACAGACCCCGGATCCACGCTGGGGGGCCTTTGCTCGAAACATTCTAGAGCGCGGT GGCCCCACCCCACGCAATGGGAATAAGTCTGACCAAGCGCACCCTCCCATCCACCCCACC AAATACACTGACAGCCTGCAGGGGGATGAGCAGCGACTGTACGAGCTCATCGTCCGCCAC TTCCTGGCCTGCTGCTCGCAGGATGCCCAGGGCCAGGAGACCACTGTGGAGATCGACATC GCGCAGGAGCGCTTCGTGGCCCACGGCCTCATGATCCTGGCCCGGAACTATCTGGACGTG TACCCGTATGATCGCTGGAGTGACAAGACCCTCCCTGTCTATGAGCAAGGCACCTGCTTT CAGCCCAGCACTGTGGAGATGGTGGACGGGGAGACCAGTCCCCCCCAGCTGCTCACCGAG GCTGACCTCATTGCCCTCATGGAGAAGCACGGCATTGGTACGGACGCCACGCACGCTGAG CACATCGAGACCATCAAGGCCCGGATGTACGTCGGGCTCACGCCAGATAAGCGCTTTCTC CCTGGGCACCTGGGCATGGGGCTCGTGGAAGGCTATGACTCCATGGGCTACGAGATGTCC AAGCCTGATCTGCGGGCCGAGCTGGAGGCTGACCTGAAGCTCATCTGCGAGGGGAAGAAG GACAAGCTGGTGGTCCTGCATCAGCAGGTGCAGAAGTACAAGCAGGTGTTCATCGAAGCC GTGGCCAAAGCCAGGAAGTTGGACGAGGCCTTGTCCCAGTACTTCGGGGCGGCTACAGAG GTAGCCCCACAGGACGCCCTCCCGGCTGTGCCCGAGCCCATCAGGAAGTGTCCTCAGTGC AGCCGGGACATGGTTCTTAAGACCAGAAAGAGTGGCGGGTTTTACCTCGGTTGCACGGGC TTCCCTGAGTGTCGGTCGGCTGTCTGGTTCCCGGACAGCGTGCTGGAGGCCAGCAGGGAT GTGAGCGTGTGCCCAGTGTGCCAGCCGCACCCAGTTTACAGGTTGAAGTTCAAGTTTAAA CGAGGCAGCCTGCCCCCAACCATGCCCCTAGAATTTGTCGGCTGCATCGGCGGGTGTGAT GAGACCCTGAGGGAGATCCTGGCCCTCAGGTTCTCCCAGGGCGCTGCCCGAGTCAGCCAG CCTGCCAGCCAGACCTCCGGCTACCTGCAGGCTAGCCAGTCCCTGAACAGAATGGACAGT GGCCAGCAGGGCCAGCCCCGGCCCCCCGTCACCAGGCCCTCGAAGGCTTTGACCAGGACT CTTCCTCCACCCGCCGTTGAGAGTGAGAGCAACTCGGTGACCTGCAACTGTGGCCAGGAG GCCCTGCTGCTCACCGTCCGGAAGGAGGGCCCCAACCAGGGCCGCCAGTTCTACAAGTGT GGCAGTGGTGGCTGCAGCTTCTTTCTATGGGCTGACAGCGGCCACCCAGGAGCCGGGCCT CCCTCCTCAGCGTCCAGACCCCCAGGCGGCCCCCTGGAACACCCCCCGGGCTCAGGGACT CTCTTGGGCGGGTCCAGCAGGCCTGGCAGTGGTGGCAGCGGTGGCGCATCCTGCCTGTGT GGCCAGCCGGCTGTCACGCGCACCGTGCAGAAGGAGGGGCCCAACCAGGGCCGCCAGTTC CACACGTGTGCCCAGCCCCGTGAGCACCAGTGCGGCTTCTTCCAGTGGGTGGACGAGAAC GTGGCCCCAGGGACCTTCGGAGGCCCCCTCTGGCCAGGAAGCAGGAGAGGAACCCAGGGG CTGGAGGCCAGAAGCAAGAGGCCTCGGGCCGGCTCCTCAGACACAGGGTCTGCAACCAAG AAACCCCGGAAGTGCAGCCTTTGCCACCAGCCTGGACATACCCGTCCCTTCTGTCCTCAG AACAGATGA 1002 111512.0 8.59 None >DNA topoisomerase MIFPAAHRALRGLPRPGTRALTRAAMEVALRGVRKILCVAEKNDAAKGIADLLSGGRMRR REGLSRFNKIYEFDYHLCGQNVTMVMTSVSGHLLAHDFRMQFRKWQSCNPLVLFEAEIEK YCPENFVDIKKTLEREAQQCQALVIWTDCDREGENIGFEVIHVCKAVKPSLQVLRARFSE ITTRAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQKIFPEVLAEQLIS YGSCQFPTLGFVVERFKAIQAFVPEVFHKIRVTHDHRDGVVEFSWKRHRLFNHTACLVLY QMCMEDPRAMVVEVRSKAKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYI SYPRTETNIFPKDLDLAALVEQQTPDPRWGAFARNILERGGPTPRNGNKSDQAHPPIHPT KYTDSLQGDEQRLYELIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDV YPYDRWSDKTLPVYEQGTCFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAE HIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKK DKLVVLHQQVQKYKQVFIEAVAKARKLDEALSQYFGAATEVAPQDALPAVPEPIRKCPQC SRDMVLKTRKSGGFYLGCTGFPECRSAVWFPDSVLEASRDVSVCPVCQPHPVYRLKFKFK RGSLPPTMPLEFVGCIGGCDETLREILALRFSQGAARVSQPASQTSGYLQASQSLNRMDS GQQGQPRPPVTRPSKALTRTLPPPAVESESNSVTCNCGQEALLLTVRKEGPNQGRQFYKC GSGGCSFFLWADSGHPGAGPPSSASRPPGGPLEHPPGSGTLLGGSSRPGSGGSGGASCLC GQPAVTRTVQKEGPNQGRQFHTCAQPREHQCGFFQWVDENVAPGTFGGPLWPGSRRGTQG LEARSKRPRAGSSDTGSATKKPRKCSLCHQPGHTRPFCPQNR AAI26548.1 A0JN73 A0JN73_BOVIN BC126547 TOP3A