| Identification |
|---|
| BMDB Protein ID
| BMDBP01129 |
| Secondary Accession Numbers
| None |
| Name
| Fructose-2,6-bisphosphatase TIGAR |
| Synonyms
|
Not Available
|
| Gene Name
| TIGAR |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Carbohydrate transport and metabolism |
| Specific Function
| Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (By similarity). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity. In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage. Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation. Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions. Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner. Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses. Involved in intestinal tumor progression. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 5 |
| Locus
| Not Available |
| SNPs
| TIGAR |
| Gene Sequence
|
>813 bp
ATGACGCGCTTCGCTCTGACCGTGGTCCGGCATGGAGAAACAAGACTTAACAAGGAGAAA
ATAATTCAAGGACAAGGAATAGATGAGCCTCTTTCAGAAACTGGATTTAAACAAGCAGCT
GCTGCTGGTATATTTCTTAAAGATGTGAAGTTTACTCATGTTTTCTCCAGTGACCTTACA
AGGACAAAGCAGACCGTGCATGGGATTTTGGAGAAGAGCAAATTTTGCAAAGATATGACA
GTAAAGTATGATTCAAGACTCCGAGAAAGGAAATATGGGGTTGCAGAAGGCAGGCCGCTG
AGCGAGCTGAGGGCCATGGCCAAAGCGGCAGGGGAGGAGTGCCCAGCATTCACACCGCCT
GGAGGAGAGACCTTGGACCAGCTGAAAAGGCGTGGGAAAGACTTTTTTGAATTTCTTTGT
CAACTGATCCTGAAAGAAGCCGGTCAGAATGAACAGTTTTCCCAGGAAGCCCCGAGCAGC
TGTCTGGAAAGTTCCCTGGCAGAGATATTTCCTTTAGGGAAGAACTGTGCCTCCACGTTT
AACTCTGACAGCGGCACCCCGGGCCTAGCAGCCAGTGTCTTAGTCGTGAGTCATGGCGCC
TACATACGAAGCCTGTTGGATTACTTTCTGACTGACCTCAAGTGCTCGTTCCCAGCGACT
CTGAGCAGGTCCGAGCTCACGTCAGTCAGCCCTAACACAGGGATGACTGTCTTCATCCTA
AACTTTGAGAAAGGAGGCAAAGGGAGACCAACTGCCCAGTGTGTGTGTGTGAACCTCCAG
GGCCACCTGGCCGGGGTGAACAAAACTCCCTAA
|
| Protein Properties |
|---|
| Number of Residues
| 270 |
| Molecular Weight
| 29467.0 |
| Theoretical pI
| 8.57 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Probable fructose-2,6-bisphosphatase TIGAR
MTRFALTVVRHGETRLNKEKIIQGQGIDEPLSETGFKQAAAAGIFLKDVKFTHVFSSDLT
RTKQTVHGILEKSKFCKDMTVKYDSRLRERKYGVAEGRPLSELRAMAKAAGEECPAFTPP
GGETLDQLKRRGKDFFEFLCQLILKEAGQNEQFSQEAPSSCLESSLAEIFPLGKNCASTF
NSDSGTPGLAASVLVVSHGAYIRSLLDYFLTDLKCSFPATLSRSELTSVSPNTGMTVFIL
NFEKGGKGRPTAQCVCVNLQGHLAGVNKTP
|
| External Links |
|---|
| GenBank ID Protein
| AAI16102.1 |
| UniProtKB/Swiss-Prot ID
| Q1JQA7 |
| UniProtKB/Swiss-Prot Entry Name
| TIGAR_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC116101 |
| GeneCard ID
| TIGAR |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
